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AAEP Update on West Nile Virus

 WEST NILE VIRUS 2000 NEWS

Horse in Massachusetts Confirmed Positive for West Nile Virus (WNV)

An additional horse has been confirmed positive for WNV infection. This is the fourth equine case of 2000 and is located in Middlesex County, Massachusetts. The is the first equine case of WNV infection ever found in 
Massachusetts.

It is the same horse that was reported as positive for WNV by the State of Massachusetts on September 1, prior to any reference laboratory confirmation.

WNV infection was confirmed today at the National Veterinary Services Laboratories (NVSL) through a WNV positive polymerase chain reaction (PCR) on brain tissue and a positive WNV IgM-capture enzyme-linked immunosorbent assay (ELISA) on serum.

The horse was a 12-year-old Arabian gelding that had onset of hind limb lameness on August 26 with slight muscle spasms and increasingly severe lameness, leading to acute recumbency and paralysis. Euthanasia was carried out on August 29.

USDA, APHIS recommendations to horse owners to reduce risk of WNV infection are to reduce mosquito breeding sites and decrease exposure to adult mosquitoes.

Additional recommendations are available from the APHIS website: http://www.aphis.usda.gov/oa/wnv/index.html 


Staten Island Man Tests Positive for West Nile Virus

August 4, 2000: New York City Health Commissioner Neal L. Cohen, M.D., announced today that a 78 year-old Staten Island man has tested laboratory-positive for West Nile virus. Serum and spinal fluid samples from the patient were received by the New York City Department of Health (NYCDOH) on 27 Jul 2000, tested for West Nile virus, and reported as positive on 28 Jul 2000. Testing at the U.S. Centers for Disease Control and Prevention (CDC) was initially equivocal on 30 Jul 2000, but repeat testing this past week was reported positive on 3 Aug 2000. Additional confirmatory test results will be available in another week. [Both cerebrospinal fluid (CSF) and sera samples were positive for IgM antibody by ELISA testing. Further confirmatory testing by plaque reduction neutralization testing (PRNT) for IgG antibody in a convalescent serum sample is pending. - Mod.JW]

The individual became ill with symptoms of meningo-encephalitis (fever, dizziness, light-headedness) on 20 Jul 2000, and was admitted to a local hospital on 22 July 2000. After one week in the hospital, the patient was released and is now at home recovering.

Dr. Cohen said, "Symptoms of West Nile virus begin between 5 to 15 days after being bitten by an infected mosquito, indicating that this individual was infected before the City implemented its recent control efforts on Staten Island. Our intensive, ongoing bird and mosquito surveillance program documented significant West Nile viral activity on Staten Island in mid-July, especially in the southern half of Staten Island where this patient lives. Spraying was conducted on 19 Jul 2000 in response to those early warnings, as well as follow-up spraying throughout Staten Island on 2 Aug 2000 as a precaution. In addition, more than 40 lakes, ponds, and marshes on Staten Island received aerial applications of larvicide on 27 & 28 July 2000, and larviciding of catch basins is ongoing. The City will continue to take action where necessary to reduce the mosquito population and minimize the potential for additional human illnesses based on positive surveillance findings in birds, mosquitoes, and humans.

"With this news of the City's first human case of West Nile virus this year, and with the information we have received over the past few weeks on the increasing numbers of dead birds confirmed to be infected with West Nile virus in New York City, all New Yorkers, particularly those on Staten Island and those more at risk for severe illness, especially the elderly, should continue to take precautions against mosquitoes. In addition, I urge all New Yorkers to help the City and each other to mosquito-proof New York City by eliminating the areas of standing water where mosquitoes may breed," Dr. Cohen said.

Staten Island Borough President Molinari said, "Our Helpline unit has fielded nearly 1,500 calls, including reports of more than 500 dead birds and over 200 standing water conditions."

For information on West Nile virus, spraying activities, or to report dead birds and areas of standing water where mosquitoes may breed, New Yorkers may call the NYCDOH's West Nile virus information line, 24 hours a day, 7 days a week, at 1-877-WNV-4NYC (1-877-968-4692).(New Yorkers who use TTY/TDD can call 212-788-4947 weekdays from 9:00 a.m. to 5:00 p.m.).

Extensive information is also included on the City's Web site at www.nyc.gov/health   


West Nile virus update: 2 new States, multiple new counties, found positive

July 30, 2000: Two counties in Massachusetts (Middlesex, Suffolk) have each had a crow confirmed as positive for West Nile virus (WNV). These are the first ever findings of WNV in that State. Both birds were initially found alive, but soon died. Three additional counties in New York (Nassau, Onondaga, Ulster) had dead wild birds confirmed as positive for WNV for the first time this year.

This brings to 13 the total number of counties in the United States with wild birds confirmed as positive for WNV since 1 May 2000. A total of 77 wild birds have been found positive.

A mosquito pool in Connecticut has also been found positive for WNV. This is the first finding of WNV in Connecticut in 2000. The pool of Culex restuans was collected on 11 July in Stamford, Fairfield County. No positive wild birds have yet been found in the State this year.

Three new counties in New York have reported positive pools of mosquitoes. New York County (Manhattan) had its first confirmed WNV finding of 2000 when Culex species collected the week of 17 July in Central Park were found positive. Pools of Culex species in Richmond (Staten Island) and Rockland counties were also reported positive this week. Suffolk County reported 17 new positive mosquito pools in the last week, 15 of which were collected on 11 July in the Lindenhurst/Babylon area.

A total of 16 counties in Connecticut (1), Massachusetts (2), New Jersey (3), and New York (10) have now had confirmed findings of WNV since 1 May 2000. Seven counties in New Jersey (1) and New York (6) have now had two or more confirmed findings of WNV-positive wild birds. Three counties in Connecticut (1) and New York (2) have detected a single WNV-positive mosquito pool. Another six counties in Massachusetts (2), New Jersey (2) and New York (2) have detected a single WNV-positive wild bird. Only four counties, all in New York (Richmond, Rockland, Suffolk, Westchester), have had both wild birds and mosquito pools found positive.
There have been no findings of WNV-positive sentinel chickens, humans, or horses in the United States since 1 May 2000.

Confirmed WNV-Positive Wild Birds, 1 May to 28 July, 2000:
Massachusetts (2 birds)
Middlesex County (1 crow)
Suffolk County (1 crow)
New Jersey (9 birds)
Bergen County (7 crows)
Hudson County (1 crow)
Middlesex County (1 crow)
New York (66 birds)
Nassau County (2 crows, 1 song sparrow)
Onondaga County (1 crow)
Queens County * (1 blue jay, 1 crow, 1 merlin)
Richmond County * (10 crows, 1 fish crow, 1 house sparrow, 1 other)
Rockland County (5 blue jays, 1 Canada goose, 27 crows)
Suffolk County (3 crows)
Ulster County (1 crow)
Westchester County (4 blue jays, 3 crows, 2 red-tailed hawks)
Part of New York City

Confirmed WNV-Positive Mosquito Pools, 1 May to 28 July, 2000:
Connecticut (1 pool)
Fairfield County (1 Culex restuans)
New York (25 pools)
New York County * (1 Culex pipiens/restuans)
Orange County (1 Aedes japonicus)
Richmond County * (2 Culex pipiens/restuans)
Rockland County (2 Culex pipiens/restuans)
Suffolk County (18 Culex pipiens/restuans)
Westchester County (1 Aedes japonicus)
· Part of New York City

(Source: Randall L Crom (Randall.L.Crom@usda.gov


On Jun 8, 2000, the New York State and New Jersey public health laboratories reported provisional findings of West Nile virus-positive dead crows to the Center for Disease Control (CDC). In New York State, the positive tests are in two dead crows that were found in Rockland County along the Hudson River on May 22, 2000. Testing by the New York State laboratory in Albany showed evidence of West Nile virus in two different tests, and virus culture results, were also positive.

The New Jersey report involves a dead crow found in Bergen County on May 30, 2000. New Jersey does more limited testing than New York State, but the bird was positive in the New Jersey laboratory in the single test that was performed. These results were confirmed by CDC on June 8, 2000.

All recent mosquito testing for West Nile virus (including New York) has been negative, and there is no known or suspected human disease. Extensive mosquito control measures continue in the region and should reduce the potential for human disease. The bird findings are likely to reflect enhanced CDC-funded surveillance in the New York area, indicating the monitoring system is working and envisioned. The system is designed to detect evidence of virus in birds or mosquitoes before there is any human disease. Continued circulation of West Nile virus is not unexpected based on the positive data from overwintering mosquitoes in the year. New York State has issued a press release announcing the findings, and is intensifying surveillance activities in Rockland and surrounding counties in order to guide any additional control measures. This press release may be accessed at http://www.health.state.nysdoh/commish/2000/crows.htm.


 The disease causes inflammation of the central nervous system, but the clinical symptoms for West Nile fever in the horse and in man often are not diagnostic. They include typical encephalitic symptoms such as disorientation, dementia and coma. In the horse, the disease can present as a weakness or ataxia of the hind limbs. The viremiaand fever that occur following initial infection in the horse often precede the neurological symptoms by several weeks; therefore, the disease can be confusing and difficult to diagnose in the horse. There is no specific treatment for West Nile fever other than lessening the severity of central nervous system disturbance. Currently, there is no vaccine for West Nile fever and routine vaccination against western and eastern equine encephalomyelitis does not confer protection against West Nile fever.





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